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Input and Output for MaLDoSS
Input
The input sequence data should be provided in single or multiple sequences in FASTA format. An example of input sequence file is
>gene1
GAGCTCACATTAACTATTTACAGGGTAACTGCTTAGGACCAGTATTATGAGGAGAATTTA
CCTTTCCCGCCTCTCTTTCCAAGAAACAAGGAGGGGGTGAAGGTACGGAGAACAGTATTT
CTTCTGTTGAAAGCAACTTAGCTACAAAGATAAATTACAGCTATGTACACTGAAGGTAGC
TATTTCATTCCACAAAATAAGAGTTTTTTAAAAAGCTATGTATGTATGTGCTGCATATAG
AGCAGATATACAGCCTAT
>gene2
ACCTTACTCGCCCCAGTCTGTCCCGACGTGACTTCCTCGACCCTCTAAAGACGTACAGAC
CAGACACGGCGGCGGCGGCGGGAGAGGGGATTCCCTGCGCCCCCGGACCTCAGGGCCGCT
CAGATTCCTGGAGAGGAAGCCAAGTGTCCTTCTGCCCTCCCCCGGTATCCCATCCAAGGC
GATCAGTCCAGAACTGGCTCTCGGAAGCGCTCGGGCAAAGACTGCG
Output
The generated output file contains the probabilities with which the putative splice sites are predicted as true by random forest. The display page contains only those sites, which are predicted as true site with probability greater than 0.5. However, the splice site with higher probability can be considered as the donor splice site with more strength. An output file obtained by using all the three encoding procedures is shown below;
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Team: Prabina Kumar Meher, Tanmaya Kumar Sahu, A. R. Rao and S. D. Wahi
Contact: meherprabin@yahoo.com, tanmayabioinfo@gmail.com, arrao@iasri.res.in |